A Nuclepore Filter Bag for Preparation of Small Biological Specimens for Scanning Electron Microscopy.

A metal impregnation method of biological specimens for scanning electron microscopy.

Blocks of the kidney and other organs of the guinea pig fixed with glutaraldehyde and treated with tannic acid and osmium tetroxide could, without any coating, be observed in the scanning electron microscope. Undesirable charging effect was inconspicuous when accelerating voltage 5-25kV was used. A scanning image of the renal glomerulus was shown and the details of the method were described. Th...

Scanning Electron Microscopy (SEM) analysis and biological control of Ixodes ricinus using entomopathogenic fungi

In the present study, pathogenicity of four native strains of Entomopathogenic fungus; Metarhizium anisopliae, was studied against adult stage of Ixodes ricinus. For this purpose a total number of 180 adult ticks were examined in triplicate. Thirty ticks for each strain and negative and positive controls were immersed in 2.4×107 fungal conidia/ml in vitro. Samples were incubated in separate Pet...

Routine preparation of seminal fluid specimens for transmission electron microscopy.

A technique is described for the routine preparation of seminal fluid specimens for transmission electron microscopy. The method is applicable to cases where examination of sperm morphology is required and can also be used for the indentification of non-spermatozoal cells and their inclusions.

Preparation of Drosophila specimens for examination by transmission electron microscopy.

There is no single, simple procedure for fixing and embedding all tissues for transmission electron microscopy (TEM). The chemistry of different cell types is to some extent unique, and this affects the way each cell type reacts to the wide array of fixatives, buffers, organic solvents, and resins used in TEM specimen preparation. A recurring theme in those organisms or cell types that are diff...

3-dimensional Electron Microscopy of Biological Specimens